Description
The KRIBIOLISA™ Double-Stranded RNA (dsRNA) ELISA employs the quantitative sandwich enzyme immunoassay technique. It is based on the use of two double-stranded RNA (dsRNA)-specific monoclonal antibodies which allows sensitive and selective detection of dsRNA molecules (>=40 bp), independent of their nucleotide composition and sequence. Antibodies to dsRNA (J2) are pre-coated onto microwells. Samples and standards are pipetted into microwells and are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated Anti-dsRNA (K1) is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of dsRNA in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm